Components

Echovirus Blend Monoclonal Antibodies - (Catalog No. 3311).One 2 mL dropper vial containing mouse monoclonal antibodies against echovirus types 4, 6, 9, 11, 30 and 34, protein stabilizer and 0.1 % sodium azide (preservative). (Anti-Echo 9 is a subclass IgG2b and the others are IgG2a.)

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Materials Required But Not Provided:
⋅ Acetone, reagent grade; stored in glass

⋅ Distilled water or deionized water

⋅ Sodium hypochlorite solution, 0.05% (1:100 dilution of household bleach)

⋅ Sterile shell vials with 12 mm coverslips containing monolayer of cell line appropriate for growth of enteroviruses

⋅ Tissue culture media such as RPMI or Eagle′s Minimum Essential Medium (EMEM) with fetal bovine serum (FBS) and antibiotics, or equivalent

⋅ Viral transport medium which is non-inhibitory to enterovirus

⋅ 0.1N NaOH

⋅ 0.1N HCl

⋅ Microscope slides, non-fluorescing

⋅ No. 1 cover slips

⋅ Negative and positive control slides

⋅ Mounting Fluid (Catalog No. 5013)

⋅ Echovirus Control Slides (Catalog No. 5074)

⋅ Anti-Mouse IgG:FITC Conjugate (Catalog No. 5008)

⋅ Normal Mouse Antibody such as (Catalog No. 5014) as negative control

⋅ Phosphate buffered saline (PBS, 0.01 M pH 7.1-7.4 with 0.085% NaCl and 0.1% sodium azide), (Catalog No. 5087)

⋅ 0.05% Tween 20 /0.1% Sodium Azide Solution (optional), (Catalog No. 5037)

⋅ Aspirator device with disposable sterile Pasteur pipettes

⋅ Centrifuge capable of 700-950 x g with biohazard buckets and adapters for shell vials

⋅ Fluorescence microscope with appropriate filter combination for FITC (excitation peak = 490 nm, emission peak = 520 nm) with 100x, 200x, 400x magnification (dry objective)

⋅ Forceps

⋅ Humid chamber

⋅ Incubator, 37 * 1*C

⋅ Syringe filter, 0.45 micron

⋅ Ultrasonic water bath

⋅ Vortex mixer or sonicator

Format: Purified

Storage and Stability

When stored at 2-8°C, the monoclonal antibody is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures. Discard any remaining reagent after the expiration date.

Warning and Precautions:

* The performance of Light Diagnostics Echovirus Blend MAb has not been determined on direct specimens.

⋅ Sodium azide, present in the reagent, may react with lead and copper plumbing to form potentially explosive metal azides. When disposing of solutions that contain sodium azide, flush plumbing with a large volume of water to prevent build-up.

⋅ Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite prior to disposal.

⋅ Avoid contact with Evans blue, if present in any reagent, as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

⋅ Do not mouth pipette reagents.

⋅ Do not allow shell vials or slides to dry at any time during the staining procedure.

⋅ Pooling or alteration of any reagent may cause erroneous results.

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

⋅ Mounting Fluid (Catalog No. 5013) contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.

⋅ Slides prepared too early (﹤25% CPE) or too late (>95% CPE) can be difficult to read and can lead to false negatives.